Proteome-wide analysis of phospho-regulated PDZ domain interactions through phosphomimetic proteomic peptide phage display
نویسندگان
چکیده
1. Department of Chemistry – BMC, Uppsala University, Husargatan 3, Box 576, 751 23 Uppsala 2. Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK 3. Laboratory of Physical Chemistry, ETH Zürich, 8093 Zürich, Switzerland 4. Institute of Biophysical Chemistry, Goethe University, 60438 Frankfurt am Main, Germany 5. Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, SE-75123 Uppsala, Sweden.
منابع مشابه
Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes.
The human proteome contains a plethora of short linear motifs (SLiMs) that serve as binding interfaces for modular protein domains. Such interactions are crucial for signaling and other cellular processes, but are difficult to detect because of their low to moderate affinities. Here we developed a dedicated approach, proteomic peptide-phage display (ProP-PD), to identify domain-SLiM interaction...
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